Efficacy of neem leaf extract against ascariasis in indigenous chicken
Jasim Uddin Khokon1*, Sharifuzzaman2, Emran Hasan Sarker1, Mohammad Afazur Rahman1, James Jony Kisku3, Mahbub Mostofa1
1Department of Pharmacology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
2Department of Parasitology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
3Department of Medicine, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
ABSTRACT
The efficacy of a medicinal plant neem (Azadirachta indica) leaves and a reference anthelmintic (piperazine) was evaluated against adult Ascaridia galli in chicken in vitro and in vivo. The aqueous extracts of neem leaves and a rage of piperazine concentrations were screened for their anthelmintic effects against Ascaridia galli in indigenous chicken. The data revealed that the aqueous extract of neem leaves showed significant anthelmintic efficacy at a dose rate of ranging from 1 mg/ml, 2 mg/ml, 4 mg/ml and 20 mg/ml against the chicken ascariasis. Shortest time of parasite paralysis was observed at dose rate 20mg/ml. Piperazine was incubated at the dose rate of 0.1, 0.2, 0.4, 0.8 and 4mg /ml to adult worms. Shortest time of parasite paralysis was observed at dose rate 4mg/ml. Using the extract of neem leaves at a dose of 1gm/kg body weight (bd. wt.) and piperazine at a dose of 200mg/kg body weight single dose for seven consecutive days showed gradual increase of efficacy up to day 14th and 21st of treatment, respectively. In post-mortem worm count neem leaves showed considerable efficacy against worm burden. The live body weight was increased significantly in chicken after administration of neem leaves extract (1 gm/kg bd.wt) and Pipervet® (200mg/kg bd.wt) in groups ‘B’ and ‘C’ respectively. But in control group ‘A’ the change of body weight was not significant. Increase values of TEC, Hb and PVC were observed in chicken after treatment with Pipervet® (200mg/kg b.wt.) and neem leaves extract (1 gm/kg bd.wt). However, the aqueous extract of neem did not show any adverse effect on haematological parameter and physiological condition of treated birds.
Key words: Ascaridia galli, neem leaves extract, chicken
*Corresponding author. Tel.: + 8801816467202
E-mail address: jkhokon@ymail.com (JU Khokon) Full Text (PDF)
