Md. Mostafizur Rahman¹, Sumana Al Mazid³, Md. Kamrul Hasan⁴, Zohirul Islam Rony⁵, Amin MM², Md. Tanvir Rahman²

¹Upazilla Livestock Office, Bahubal, Habiganj, Bangladesh
²Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
³Upazilla Land Office, Bahubal, Habiganj, Bangladesh
⁴Eskayef Bangladesh Limited, Bangladesh
⁵Department of Biochemistry and Food Analysis, Patuakhali Science and Technology University, Patuakhali-8602, Bangladesh

Present study was carried out to study the immunogenicity of a commercial available formalin-killed alum-precipitated Salmonella pullorum vaccine manufactured by livestock and poultry vaccine research and development centre (LPVRDC) at the Department of Microbiology and Hygiene, BAU. Mymensingh. Vaccination was carried out on Shaver brown chicken (parent stock) of the Phenix Poultry Ltd. Located at Bhaluka, Mymensingh. Birds belonging to the group A (n=11,000) were used for vaccination, while birds of group B (n=10) was used as unvaccinated control group. Each bird was vaccinated intramuscularly at 65 days of age with a subsequent booster dose after 35 days of primary vaccination. Following primary vaccination the mean value of Passive Hemagglutinating (PHA) antibody titers of vaccinated birds, at 15, 30 and 35 days were 116.00±12.00, 96.00±8.26 and 88.00±11.71 respectively. While, the subsequences mean value of PHA antibody titers of vaccinated birds at 15 and 30 days following the booster vaccination were 128.00±10.12 and 108.00±7.66, respectively. The mean PHA antibody titer in chickens of unvaccinated control birds were ≤ 4.25±0.51. The findings demonstrated that the level of PHA titer after primary vaccination decreased immediately; however, after booster vaccination the level of titer of PHA antibody increased and sustained. Present study indicated that the S. pullorum killed vaccine prepared at by LPVRDC; significantly (p<0.01)) increased the level of PHA antibody in  vaccinated chickens compared to unvaccinated control as determine by the PHA test.

Keywords: Salmonella pullorum, killed vaccine, Immunogenicity, PHA antibody titers, On-farm study, Shaver Brown Chicken.

Corresponding author.
E-mail address:  tanvirahman@gmail.com (MT Rahman)

How to cite this article: Rahman MM, Mazid SA, Hasan MK,  Rony ZI,  Amin MM and Rahman MT (2016). Immunogenicity of Salmonella pullorum killed vaccine in selected breeder flock. International Journal of Natural and Social Sciences, 3(1): 01-04.

Ayesha Siddiqua¹, Md. Nazrul Islam¹, Md. Mahfuzur Rahman¹, M.M.A. Yahia Khandoker², Abu Saleh Mahfuzul Bari¹

¹Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh
²Department of Genetics and Animal Breeding, Bangladesh Agricultural University, Mymensingh, Bangladesh

The study was conducted at the nuclear breeder farm of black Bengal goat belongs to the Department of Pathology, Faculty of Veterinary Science and Department of Animal breeding and Genetics, Bangladesh Agricultural University, Mymensingh from March to September, 2008. A total of 10 breeding bucks were used as experimental animal. The aim of the investigation was to analyze of goat semen by using Eosin-Nigrosin stain and Rose Bengal stain under differential interference microscope. The study included investigation of the age in month, ejaculation volume (ml), mass activity of the semen, spermatozoa motility (%), viable % spermatozoa and normal spermatozoa, and morphologically abnormal sperm especially in head that included the acrosome, midpiece, and tail. Semen was collected twice in a week from each buck by using Artificial Vagina method. Age of bucks were 14.9±4.1 months, ejaculate volume of semen was 0.5±0.3 ml, motility was 80.8±3.5 (%), mass activity was 4.The percentage of viable spermatozoa was 89.6±5.1 .The percentage of spermatozoa with midpiece was 6.9±2.5 and tail abnormalities was only 7.1±2.6 and that of with head abnormalities was 2.5±1.7. The morphologically normal spermatozoa were 90.84%.

Keywords: Black Bengal goat, semen, abnormalities, spermatozoa.

Corresponding author.
E-mail address:  ayeshamahbub1012@gmail.com (A Siddiqua)

How to cite this article: Siddiqua A, Islam MN, Rahman MM, Khandoker MMAY and ASM Bari (2016). Abnormalities of semen of Black Bengal Goat in Bangladesh. International Journal of Natural and Social Sciences, 3(1): 05-09.

Md Masudur Rahman Khalil^1,2, Tushar Pudder³, Md Gahangir Alam⁴, Goutom Chandra Mondol⁴,  Bishwajit Modak⁵, Ashish Kumar Das³, Mir Himayet Kabir¹

¹Centre for Infectious Diseases Division, ICDDR’B, Mohakhali, Dhaka-1212, Bangladesh
²Department of Microbiology and Biotechnology, Jagannath University, Dhaka-1100, Bangladesh
³Department of Microbiology, Stamford University, Dhaka-1209, Bangladesh
⁴Department of Microbiology, Gono Bishwabidyalay (University), Dhaka-1344, Bangladesh
⁵Bangladesh Institute of Research & Rehabilitation in Diabetes, Endocrine and Metabolic Disorders, Dhaka-1000, Bangladesh

Tea is a popular drink in Bangladesh and taken by a large number of people. Many people are engaged in small business for selling food and drinks on the roadsides as mobile and permanent vendors. This study was carried out to determine the microbiological quality of tea vendors in Saver, Dhaka. A total of 10 street tea vendors (5 permanent and 5 temporary) were randomly selected for this study. Tea cup, vendor hand and water samples were collected from each tea vendors. All samples were assessed for the presence of total bacterial count, total Enterobacteriaceae, total coliform and total Staphylococcus aureus. The presence of Salmonella, Shigella and Vibrio species were determined following appropriate enrichment and culture method. Cup and hand samples collected from mobile vendors showed significantly higher count of total heterotrophic bacteria (p<0.05). Cup swabs also showed significantly higher count of Staphylococcus aureus in mobile vendors than permanent ones (p<0.05). There was no significant differences between the counts of total Enterobacteriaceae, coliforms and Staphylococcus aureus in mobile and permanent vendors (p>0.05). Salmonella and Shigella could not be detected in any of the samples. Proportion of Vibrio spp. were 20% higher in cup and hand samples collected from mobile vendors as compared to the other group. All vendors did not have adequate knowledge and training on food safety and hygiene which is also reflected by poor microbiological quality of vendor samples. Contamination of tea vendor samples with pathogenic and indicator bacteria indicate their poor quality and unacceptability as drinks. This could be a threat to the consumer’s health and require immediate attention in order to control any outbreak of food and water borne diseases.

Keywords: Bacteria, tea, vendors, Bangladesh.

Corresponding author.
E-mail address: masudurrahaman@hotmail.com (MMR Khalil)

How to cite this article: Khalil MMR, Pudder T, Alam MG, Mondol GC,  Modak B, Das AK and Kabir MH (2016). Microbiological quality of tea vendors at Dhaka, Bangladesh. International Journal of Natural and Social Sciences, 3(1): 10-17.

Md. Mahfuzur Rahman¹, Ayesha Siddiqua¹, Md. Nazrul Islam¹, M.M.A. Yahia Khandoker², Abu Saleh Mahfuzul Bari¹

¹Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh
²Department of Genetics and Animal Breeding, Bangladesh Agricultural University, Mymensingh, Bangladesh

The objective of this study was to investigate the incidence of different diseases in a bio-secured goat farm at Bangladesh Agricultural University campus, Mymensngh. The diseases were recorded mainly on the basis of history, clinical signs and examination of organism. Higher incidence (%) of diseases was found for diarrhoea 17.4 followed by fever and anorexia (16.5), pneumonia (13.8), bloat (10.6), contagious ecthyma (8.3) and mixed parasitic infestation (7.0). Incidence of diseases had higher in adults (>1year) 40.0% than young goats (7-12months) 31.7% and kids (<6 months) 28.4, respectively. Higher incidences of diseases were found in rainy season (49.6%) and then winter (30.4%) and dry period (19.6%). The higher incidence of diseases in intensive rearing systems possibly due to increased stresses on animal, to which they responded by higher diseases incidence. Moreover, these animals were purchased from farmer level where they have lack of knowledge for preventive health care. This group of animals may act as carrier of many diseases and it might be the causes of higher incidence of diseases.

Keywords: Diseases, nuclear breeding flock, biosecured farm, goat, Bangladesh.

Corresponding author.
E-mail address: mahfuzdtm.ag@gmail.com (MM Rahman)

How to cite this article: Rahman MM, Siddiqua A, Islam MN, Khandoker MMAY and ASM Bari (2016). Diseases in nuclear breeding flock of Black Bengal Goat. International Journal of Natural and Social Sciences, 3(1): 18-24.

Nigar Sultana Meghla¹, Maruf Hossain¹, Badrul Alam¹, Lovely Rani Paul¹, Nigarin Sultana¹, Ashish Kumar Das², Md. Bakhtiar Lijon²

¹Department of Microbiology, Jessore University of Science and Technology, Jessore-7408, Bangladesh
²Modern Food Testing Laboratory, Chittagong City Corporation, Chittagong, Bangladesh

Since the beginning of human civilization, medicinal plants have been used by mankind for its therapeutic value and even in modern times have formed the basis of many pharmaceuticals in use. The aim of the present study was to evaluate the antimicrobial activity of different extracts of Piper betle, Boerhavia diffusa, Oxalis corniculata, Centella asiatica, Camellia sinensis, Curcuma longa and Allium cepa traditionally used in Bangladesh. The antimicrobial activity of these different solvent extracts were tested against Gram-positive (Staphylococcus aureus) and Gram-negative bacterial species (Escherichia coli and Salmonella spp.) by observing the zone of inhibition by disc diffusion method. The tested bacterial species were isolated from hospital waste water sample and fresh poultry meat samples where Chloramphenicol was used as standard for antibacterial assay. The results of the study revealed that the ethanolic, methanolic, and aqueous extracts of almost all the seven herbal species successfully showed to be effective against the tested Gram-positive and Gram-negative bacteria with only negative result observed in case of Centella asiatica. The methanolic and water extract of Boerhavia diffusa was found to be slightly effective producing a shorter zone of inhibition. Among these medicinal plant products, Camellia sinensis and Piper betle was found to be more effective against the tested bacterial species where methanolic extracts showed maximum zone of inhibition against Gram-positive (S. aureus with zone of inhibition of 12 mm and 11 mm respectively) and Gram-negative bacteria (e.g. Salmonella spp. with  zone of inhibition of 16 mm and 14 mm). The result confirmed the presence of antibacterial activity of Piper betle extract and other five medicinal plant extracts against various human pathogenic bacteria which should be further tested for other pathogenic multi drug resistant bacteria and could be used as an effective biocontrol agent in future.

Keywords: Medicinal plants, antibacterial property, Gram positive bacterium, Gram negative bacterium

Corresponding author.
E-mail address: lijonmicro2014@gmail.com (MB Lijon)

How to cite this article: Meghla NS, Hossain M, Alam B, Paul LR, Sultana N, Das AK and Lijon MB (2016). Evaluation of some medicinal plants against Escherichia coli, Salmonella spp. and Staphylococcus aureus. International Journal of Natural and Social Sciences, 3(1): 25-31.

Md. Montasir Mamun¹, Abu Saim Al Salauddin¹, Md. Farhad Hossain¹, Marya Afrin², Md. Asaduzzaman¹, Md. Ehsanul Kabir³, Shahin Mahmud⁴

¹Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh
²Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh, Bangladesh
³Department of Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh
⁴Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail, Bangladesh

Asthma is one of the major public health problems worldwide. There are few data available on the prevalence of asthma in adult population in Bangladesh. Despite of these data, prevalence of asthma among the undergraduate students of a specific university in Bangladesh has not been studied before. Students from different geographical location come to study in Bangladesh Agricultural University, Mymensingh. It is noticed that few students are found to be suffering from asthma. Therefore, this survey was planned to estimate the prevalence of asthma and to find out the probable risk factor among the undergraduate students of Bangladesh Agricultural University. This questionnaire-based study was carried out in every residential hall of Bangladesh Agricultural University. A room-to-room visit was performed and relevant information was recorded through questioning the undergraduate students by undergraduate students of Faculty of Veterinary science. Total 1371 students were surveyed. Overall prevalence of asthma was recorded as 10.7% (n= 146/1371). The prevalence of asthma among male was 11.7 % (n= 93/146) and female was 9.2% (n= 53/146). It was seen that a newly admitted student as well as level-1 student is less likely affected by asthma (7.5%) whereas a final year student; level-4 or above is affected in higher percentage (12.2%). A significant association of prevalence of asthma was found with dust allergy, cold allergy, food allergy and asthma patient in family (p<0.05).

Keywords: Asthma, Prevalence, Risk factor, Undergraduate students, Bangladesh.

Corresponding author.
E-mail address: muntasirsaikat@gmail.com (MM Mamun)

How to cite this article: Mamun MM, Salauddin ASA, Hossain MF, Afrin M, Asaduzzaman M, Kabir ME and Mahmud S  (2016). Prevalence of asthma and its associated factors among the undergraduate students of Bangladesh Agricultural University. International Journal of Natural and Social Sciences, 3(1): 32-36.

Asaduzzaman¹, Md. Golam Sorwar², Md. Ashraf Ali³, Mohummad Muklesur Rahman⁴, Sharifuzzaman¹, Md. Shahiduzzaman¹

¹Department of Parasitology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
²Department of Pharmacology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
³Department of Pathology and Parasitology, Jhenidah Government Veterinary College, Jhenidah, Bangladesh
⁴Veterinary Surgeon, District Veterinary Hospital, Gazipur, Bangladesh

The protozoa under the genus cryptosporidium are zoonotic apicomplexan obligate intracellular parasites which infect the intestinal epithelium of diverse mammals including humans, and cause a diarrheal disease, cryptosporidiosis. Cryptosporidiosis is a zoonotic disease and a major threat to human health due to the omnipresent distribution of Cryptosporidium species affecting humans and animals and due to the resistance of the oocysts to harsh environmental conditions and various disinfectants. Vegetable act as a great reservoir for contamination and a potential transport medium of the pathogens. The study was aimed to isolate and identify Cryptosporidium oocysts in vegetable samples in Bangladesh. Among various types of vegetables, red spinach, fresh coriander, water spinach and jute leaf samples were collected from different areas of Mymensingh and Sherpur districts of Bangladesh. To detect Cryptosporidium, the samples were assessed by microscopy, using the conventional Ziehl-Neelsen staining method. Overall 10 (16%) of 63 samples examined by microscope, were tentatively found as positive for cryptosporidium oocysts. Altogether, 6 (15%) of 40 samples, examined by microscopy, were tentatively detected as positive for Cryptosporidium oocysts in Mymensingh district. In Sherpur district, 4 (17%) of the 23 samples examined by microcopy were found as positive. The results represent the presence of Cryptosporidium in different types of sources of vegetables in both Mymensingh and Sherpur districts. However, further studies are needed to explore the molecular characters at genotype and subtype level for better understanding the transmission dynamics of the parasite.

 Keywords: Cryptosporidium, oocyst, vegetables, Bangladesh.

Corresponding author.
E-mail address: szaman@bau.edu.bd (M Shahiduzzaman)

How to cite this article: Asaduzzaman, Sorwar MG, Ali MA., Rahman MM, Sharifuzzaman and Shahiduzzaman M (2016). Investigation of Cryptosporidium oocyst in vegetables in Bangladesh. International Journal of Natural and Social Sciences, 3(1): 37-41.

Abu Syed Md. Jobaydul Alam², Mst. Mahabuba Khatun², Shakil Arvin Zomo², Nizamul Haque Patwary², Md. Enamul Haque¹

¹Department of Agricultural Extension and Rural Development, Bangabandhu Sheikh Mujibur Rahman Agricultural University,  Gazipur-1706, Bangladesh
²Department of Agricultural Extension, Khamarbari, Dhaka, Ministry of Agriculture, Bangladesh

The main purpose of the study was to determine the impact of Food Security progarm on crop production. Mithapukur and Sadar upazilas of the Rangpur distric were the locality of the study. Sixty respondents were selected as the sample for the study by using simple random sampling technique. Data were collected with help of an interview schedule. The entire process of data collection took 30 days from June 25 to July 24, 2007.The impact of food security project was measured on the bsis of extent of change occured in use of optimum irrigation water, use of balanced fertilizer, credit received and utilization, crop production and annual income of the resondents. Most of the resondents were young aged having secondary and above level, possessed small farm, medium extension contact and partially positive attitude towards food security project. Majority of the respondents used optimum irrigation water and balanced fertilizer for rice, potato and maize cultivation after involvement in food security project as compared to their involvement before. Farmers also got greater credit facilities after involvement with food security project. Hence, after involvement in food security project, production of rice, potato and maize incresed significantly which ultimately incresed farmer’s annual income, education, farm size, farming experience, training exposure, extension contact.

Keywords: Food security, crop production, Bangladesh.

Corresponding author.
E-mail address: shaheenbinod@gmail.com (ASMJ Alam)

How to cite this article: Alam ASMJ, Katun MM, Zomo SA, Patwary NH and Haque ME (2016). Impact of food security project on crop production in Bangladesh. International Journal of Natural and Social Sciences, 3(1): 42-51.

Sudeb Sarker¹, Nurjahan Begum¹, Anita Rani Dey¹, Partha Pratim Roy¹, Saroj Kumar Yadav²,  MM Hussain Mondal¹

¹Department of Parasitology, Bangladesh Agricultural University, Mymensigh-2202, Bangladesh
²Department of Parasitology, Chittagong Veterinary and Animal Sciences University, Chittagong, Bangladesh

The aim of the study is to investigate the prevalence of endoparasites of pigs in Bangladesh. The study was conducted in different areas of Chittagong district from May to August, 2014.  Fecal samples were collected from 86 pigs and examined by using direct smear and Stoll’s ova counting techniques. All animals were found infected with one or more species of parasites. Five species were identified, namely Schistosoma sp (24.41 %), Fasciolopsis sp (66.27%), Ascaris sp (70.93%), Strongyloides sp (38.37%) and Balantidium sp (52.32%). The prevalence of gastrointestinal parasites in relation to age and sex were examined. Data showed that there was no significant (p>0.05) difference among piglets, growers and adults. No significant difference was observed in infection rates between male and female (p>0.05). EPG/CPG/OPG was calculated and ranged from 100-1500. It is concluded that gastrointestinal parasites were prevalent in pigs in the study area. However, further investigation is needed to find out possible impact of parasitic infestations of pigs on public health.

Keywords: Prevalence, parasitism, endoparasites, pig, Bangladesh.

Corresponding author.
E-mail address: sarkersudeb@gmail.com (S Sarker)

How to cite this article: Sarker S, Dey AR, Begum N, Roy PP, Yadav SK and Mondal MMH (2016). Prevalence of endoparasites in pig in Chittagong, Bangladesh. International Journal of Natural and Social Sciences, 3(1): 52-58.

Partha Pratim Roy¹, Nurjahan Begum¹, Anita Rani Dey¹, Sudeb Sarker¹, Hiranmoy Biswas², Thahsin Farjana¹

¹Department of Parasitology, Bangladesh Agricultural University, Mymensigh-2202, Bangladesh
²Upazilla Livestock Officer, Char fashion, Bhola, Bangladesh

A study was conducted to determine the prevalence of gastrointestinal (GI) parasites in buffaloes at Mongla in Bagerhat of Bangladesh from July to November, 2014. A total of 213 buffaloes were examined for GI parasites by using Stoll’s ova counting technique. The data demonstrated that all animals were found to be infected with one or more gastrointestinal parasites. Five species of gastrointestinal parasites were identified. Among them two species were trematodes, Fasciola gigantica (24.41%) and Amphistomes (78.40%); two species were nematodes, Haemonchus contortus (29.58) and Toxocara vitulorum (18.78%); one species was protozoa, Balantidium coli (80.28%). No cestode was detected. Mixed gastrointestinal parasitic infection was common. Prevalence of gastrointestinal parasites in relation to age, sex, nutritional status and season were studied. All age groups of buffaloes were susceptible to infection. Males and females were equally infected with gastro-intestinal parasites. It was also detected that, nutritional status of buffaloes had no significant (p>0.05) effect on gastro-intestinal parasitic infection. There was no seasonal variation in case of parasitic infections. In this study, EPG/CPG (Egg/cyst Per Gram of Feces) was determined that ranged from 100-2100 among the identified parasites. The study revealed that the prevalence of gastro-intestinal parasites in buffaloes is very common.

Keywords: Prevalence, gastrointestinal parasites, buffalo, Bagerhat, Bangladesh.

Corresponding author.
E-mail address: partha.dvm@gmail.com (PP Roy)

How to cite this article: Roy PP, Begum N, Dey AR, Sarker S, Biswas H and Farjana T (2016). Prevalence of gastrointestinal parasites of buffalo at Mongla, Bagerhat. International Journal of Natural and Social Sciences, 3(1): 59-66.

Debashis Kumar Mondal¹, MNS Mamun Siddiky², Mritunjoy Paul³

¹Bangladesh Fisheries Research Institute, Freshwater Sub-station, Jessore, Bangladesh
²Bangladesh Fisheries Research Institute, Brackishwater Station, Khulna, Bangladesh
³Bangladesh Fisheries Research Institute, Freshwater Station, Mymensingh, Bangladesh

An 8 week feeding trial was conducted in synthetic hapa in pond to asses and compare the nutritive value of three commercial fish feed through chemical analysis and their effect on the growth and survival of tilapia (GIFT strain, Oreochromis niloticus) fry (0.008±0.00g). In the rearing pond, overall water quality parameters were at the acceptable level. Three commercial fish feed, were collected from market and proximate composition such as moisture, crude protein, crude lipid, ash and fiber were analyzed in the laboratory. This experiment was designed with three treatments each having two replications. Three different feed viz, National feed (T₁), Quality feed (T₂), Mega feed (T₃) were supplied four times a day. Growth of tilapia fry under treatment T₃ in terms of weight was significantly (p<0.01) higher (7.23±0.3866 g) than those of other treatments. Treatment T₁ showed the significantly (p<0.01) lowest (4.097±0.1745 g) growth performance among the three treatments. The highest survival rate (90.275±0.460%) was obtained with treatment T₃. Comparatively better growth and survival rate of fry was obtained with Mega feed. From the result of both nutritive value and growth performance Mega feed can be the recommended feed for the growth and production of tilapia fry.

Keywords: Tilapia fry, fish feeds, growth performance, Bangladesh.

Corresponding author.
E-mail address: debbfri@gmail.com (DK Mondal)

How to cite this article: DK Mondal, MNSM Siddiky and M Paul (2016). Evaluation of nutritive value of three commercial fish feed and their effect on the growth and survival of tilapia (Gift Strain, Oreochromis niloticus) fry. International Journal of Natural and Social Sciences, 3(1): 67-72.

Aovijite Bosu¹, Monoranjan Das², Sajjad Hossain², Md. Moniruzzaman¹

¹Bangladesh Fisheries Research Institute, Mymenisngh-2201, Bangladesh
²Department of Aquaculture, Bangladesh Agricultural University, Mymensingh – 2202, Bangladesh

A study was carried out to assess the quality of fish feed used in different tilapia (Oreochromis niloticus) farms of Trishal and Fulpur Upazila in Mymensingh District. A total of 8 tilapia feeds viz. as C.P, Nourish, Quality Feed, Provita, Paragon, Mega, Fortune and a Farm made feed were collected during February to April 2014. The samples were analyzed for proximate composition in the Fish Nutrition Laboratory, Department of Aquaculture, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh. Growth data were collected from farms record book and analyzed later on. The moisture content of feeds varied from 13.96 to 10.57%. Lipid content varied from 10.38 to 2.69%. The highest 33.60% protein was observed in Quality Feed and the lowest (25.9%) observed in Fortune Feed. The moisture content, Crude lipid, Crude fibre, Carbohydrate and Ash were varied from 13.96 to 10.57%, 6.50 to 4.20%, 40.45 to 24.84% and 14.09 to 8.50%, respectively. The maximum final weight 68.96g was observed in C.P Feed whiles the lowest 21.05g for Fortune Feed. The highest weight gain (63.39g) exhibited by C.P Feed whereas the lowest (19.11g) for Fortune Feed. The highest SGR (70.43%) exhibited by C.P Feed and lowest SGR (21.23%) exhibited by Fortune Feed. The maximum Production 53.17kg/dec./90 days exhibited by C.P Feed while the lowest 26.25kg/dec./90 days exhibited by Fortune Feed. The best FCR observed was 2.97 by feeding C.P Feed and the worst FCR was observed in 4.12 by feeding Mega Feed. Survival rate was varied from 96.39 to 78.36%. The results of the present work will be very much helpful to fish farmers for feed selection and bargain with the feed traders to select suitable feed for their fish to ensure profitable aquaculture operation.

Keywords:Feed. Quality Assessment, Proximate Composition,FCR, SGR, Tilapia.

Corresponding author.
E-mail address: ovi_bosu08@yahoo.com (Aovijite Bosu)

How to cite this article: Bosu A, Das M, Hossain S and Moniruzzaman M (2016). Evaluation of commercial feed on growth performance of Tilapia (Oreochromis niloticus) in Mymensingh. International Journal of Natural and Social Sciences, 3(1): 73-82.

Bipul Chandra Roy¹, Aovijite Bosu², Md. Naim Uddin³, Md. Moniruzzaman²

¹Department of Fisheries Technology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
²Bangladesh Fisheries Research Institute, Mymensing-2201, Bangladesh

The goal of this study was to monitor the quantitative bacterial flora in the freshwater prawn in fresh and in frozen storage conditions for 1 year. This study was also conducted for bacteriological analysis of water and sediment sample to determine the physico-chemical parameters of two prawn ghers from Rupsha and Batiaghata upazilla under Khulna district. The ghers were designated as gher-1 and gher-2 respectively. Duration of the study was April, 2014 to April, 2015. The study included physico-chemical parameters like water temperature, dissolve oxygen (DO), pH and salinity of gher water; bacteriological study like aerobic plate count and presence of enteric bacteria in gher water, sediment and prawn samples in fresh and frozen storage condition. Sampling was done by one month interval with three replication for bacteriological analysis. The samples were collected from three different corner of each gher. Mean water temperature (°C), dissolve oxygen (mg l-1), pH and salinity (ppt) in gher 1 and 2 were 27.85, 27.95; 4.90, 5.0; 6.8, 6.7 and 9.5, 8.5, respectively. In gher-1, total viable bacterial count in water ranged from 8.34×103 to 2.45×104; in sediment ranged from 7.17×108 to 1.51×109. In gher-2, it ranged from 7.75×104 to 1.43×105 in water; 9.87×108 to 8.59×109 in sediment. On the other hand, In fresh and frozen fish, aerobic plate count (APC) ranged from 3.2×107 to 1.4×104 CFU g-1 in the freshwater prawn collected from gher 1 and 7.48×107 to 2.5×104 CFU g-1 in the freshwater prawn from gher-2. APC for all the groups of fish decreased around 2-log cycles after 1 months frozen storage; thereafter, counts slowly declined during frozen storage for 1 year. Gram-negative rods (77%) dominated. Enteric bacteria were also present in both ghers. But in the frozen storage condition, Enteric bacteria were not detected in any of examined prawn samples. The presence of enteric bacteria in the gher waters, sediments and fresh prawn samples suggested that waters were polluted by mammals, bird faeces and other outer sources.

Keywords:Quantitative Bacterial Flora, Freshwater Prawn, Aerobic Plate Count, Enteric Bacteria, Fresh and Frozen Storage.

Corresponding author.
E-mail address: ovi_bosu08@yahoo.com (Aovijite Bosu)

How to cite this article: Roy BC, Bosu A , Uddin MN and Moniruzzaman M (2016). Quantitative analysis of bacterial flora of freshwater prawn (Macrobrachium rosenbergii) in fresh and frozen storage condition. International Journal of Natural and Social Sciences, 3(1): 83-92.