Isolation and identification of bacteria from antibiotic industry liquid waste outlet
Md. Masudur Rahman Khalil1*, Md. Mehdi Hasan2, Md. Rowfur Rahman2, Sharmin Islam3, Ashish Kumar Das3, Md. Farukh Faisal Ashrafi3, Md. Nazmul Hossain4
1Department of Microbiology and Biotechnology, Jagannath University, Dhaka-1100, Bangladesh
2Department of Microbiology, Gono Bishwabidyalay (University), Dhaka-1000, Bangladesh
3Department of Microbiology, Stamford University Bangladesh, Dhaka-1209, Bangladesh
4Department of Microbiology, Prime Asia University, Dhaka-1000, Bangladesh
ABSTRACT
Antibiotic pollution due to poor or inadequate waste management practice by manufacturing industries, hospitals, poultries and veterinary may make environmental microbes resistant against the antibiotics. Antibiotic plant waste outlet connected with pond in Bangladesh may contain active antibiotics or ingredients which cause resistance to the dweller bacteria due to selective pressure. In order to address the hypothesis bacterial total counts, resistance counts and resistance pattern of bacteria of Gonoshasthay Kendray (GK) pond was analyzed. Ampicillin was used to count total resistant bacteria because this antibiotic is produced in the GK antibiotic plant. The isolates (Pseudomonas, Staphylococcus, Streptococcus, and Alkaligens) identified on the basis of gram staining, morphology and biochemical tests. Thirty individual colonies were isolated from the resistance count plate to study the resistance pattern. Amoxicillin, Tetracycline, Streptomycin, Penicillin-G, Ciprofloxacin, Cefalexin, Azithromycin, Gentamycin were used as indicator antibiotic. The rate of resistance among the isolates for Amoxacillin, Ampicillin, Ciprofloxacin, Streptomycin and Penicillin-G were 100%, Azithromycin 12.5%, Gentamycin 12.5%. Only Tetracycline and Cephalexin were 100% sensitive. The results evident that antibiotic plant pond content multidrug resistance bacteria which indicating the plant liquid waste carry active antibiotics or ingredients.
Key words: Antibiotic resistant, bacteria, waste outlet, Bangladesh
*Corresponding author. Tel.: +88 01915614377
E-mail address: masudurrahaman@hotmail.com (MMR Khalil) Full Text (PDF)
